Fig. 4. An active microtubule-dependent transport is required to maintain EJC localization around centrosomes.

eIF4A3 antibody-stained quiescent RPE1 cells treated with either DMSO or Nocodazole (a), either DMSO or CiliobrevinD (b), and chilled quiescent cells subjected to a microtubule regrowth assay (c). Centrosomes were labeled by FOP antibody and primary cilia and centriole were stained by poly-glutamylated tubulin (PolyGlu-Tub) antibody (a, b). Microtubules were stained by ß-tubulin (ß-tub in figure) antibody (c). Nuclei were stained by Hoechst. Lower panels show enlarged images marked by white dashed square in the upper panel. Scale bars in the upper and lower panels are 10 and 3 μm, respectively. Quantification of fluorescence intensities of eIF4A3 (d–f) were performed as described in the legend of Fig. 1. The average fluorescence intensities for eIF4A3 in DMSO treated cells (d, e) or in pre-incubated quiescent cells (f) are set to 1.0. Boxes represent values between the 25th lower and 75th higher percentile, and the red lines mark the median. Whiskers above and below correspond to 0.35th lower and 99.65th higher percentile, respectively. n.s. P > 0.05, *P ≤ 0.05, and ****P ≤ 0.0001, two tailed Mann–Whitney test. The number of cells analyzed in three independent experiments is provided (d–f). Pre designates pre-incubation. Source data are provided as a Source Data file.