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. 2021 Mar 1;11:4903. doi: 10.1038/s41598-021-84036-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Quantification and comparison of collagen, smooth muscle cells, and interstitial spaces between the murine endo- and ectocervix by using Visiopharm Software on Masson Trichome stained cervical sections. NP, GD15, GD18, GD19, and laboring cervixes were all combined in the analysis using Visiopharm Software Engine (version 6.6.1.2572) to evaluate overall trends in the regions of interest. Data in the bar graphs are presented as mean ± SEM. Proportion of collagen (A), smooth muscle cells (B), and interstitial spaces (C) were calculated as the area of interest (e.g. collagen, SMCs or interstitial space) divided by the total area of the endocervix or ectocervix. Endocervix has a significantly lower amount of collagen, with a significantly higher amount of SMCs than the ectocervix. There is no significant difference in the interstitial spaces between ectocervix and endocervix (p = 0.31). Statistical significance was determined by t-test. *p < 0.05, **p < 0.01, ***p < 0.001. (n = 15/region).