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. 2021 Mar 1;12(3):225. doi: 10.1038/s41419-021-03498-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a, b Membrane properties of an mCherry⁺ astrocyte recorded in an acute dorsal spinal cord slice prepared from a WT mouse infected with the control virus AAV–mCherry at 30 DPI. Membrane currents (a) and voltages (b) were recorded in voltage- and current-clamp modes, respectively, in responses to the step current or voltage commands. c, d Membrane functions of iN cells (mCherry⁺) in slices of the dorsal spinal cord that were prepared from WT mice infected with AAV–Neurog2/mCherry at 30 DPI. Membrane currents (c) and membrane voltages (d) were recorded in voltage- and current-clamp modes, respectively. e The comparison of membrane capacitance among AAV-mCherry-infected cells (red), local neurons (purple), and AAV-Neurog2/mCherry-infected cells (light blue). A one-way ANOVA revealed a significant effect of group (F[2,31] = 48.02, p < 0.001), followed by Tukey’s multiple comparison test. f The comparison of input resistance among AAV-mCherry-infected cells, local neurons and AAV-Neurog2/mCherry-infected cells. A one-way ANOVA revealed a significant effect of group (F[2,31] = 11.54, p < 0.001), followed by Tukey’s multiple comparison test. g, h Two kinds of representative traces of APs induced by depolarization currents of Neurog2-iN cells (mCherry⁺) in the slice of spinal cord. i The comparison of AP amplitude (mV) of recorded cells between local neurons and Neurog2-iN cells. Statistical analysis was performed using the two-tailed t-test method. j Schematic showing relative positions of recorded mCherry⁺ cells. D, V, R, and C in (j) represent dorsal, ventral, rostral, and caudal, respectively. k The table is a summary of inputs from DRG in 51 recorded mCherry⁺ cells from 5 mice. l Typical traces of C-fiber-evoked mono-C and poly-C EPSCs onto mCherry⁺ cells. m Typical traces of Aδ-fiber-evoked EPSPs and APs showing Aδ fiber inputs onto mCherry⁺ cells. *** Represents p < 0.001, n.s. denotes not significant.