Fig. 5.

Pharmacological inhibition of SIRT6 activates AKT/CRMP2 in primary neurons. a Primary hippocampal neurons were treated for 6 h with respectively 100 μM OSS_128167 and 50 μM LY294002, followed by Western blotting. *P < 0.05, **P < 0.01 compared with negative control (NC). b Primary neurons were pretreated with OSS_128167 and 200 μM FA, followed by Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001 compared with NC. c Primary neurons were pretreated for 24 h with different concentrations of FA, followed by Western blotting. **P < 0.01, ***P < 0.001 compared with 0 μM. d Primary neurons were incubated for 24 h with FA and 100 μM resveratrol, followed by Western blotting. *P < 0.05, **P < 0.01, ***P < 0.001 compared with resveratrol (Res). e HT-22 cells were transfected with His-SIRT6, and then incubated with FA, followed by Western blotting. *P < 0.05 compared with FA + His. About all data are expressed as mean ± SEM (n = 3)