Fig. 1.

US stimulation system and live cell calcium fluorescence imaging system. (A) One self-made steel cell holder and coverlid was designed for US stimulation, in which a 2 MHz ultrasonic transducer was fixed on the wall of holder. A 2.0 × 3.5 cm hole which can be cover by a glass coverlid was design in the middle to allow the light through the cell holder. Cells can be cultured on the glass coverlid and be irradiated by US. (B) Cell holder and coverlid can be built into a fixture with the cultivated cells and placed on a confocal microscope, thus building a live cell calcium fluorescence imaging system. (C) The diagram of whole US-stimulation live cell calcium fluorescence imaging system. (D) Characterization of US waveforms. (E) The acoustic field distribution scanned by acoustic field scanning system.