Fig. 2.

The generation of Metrnl global knockout mice (Metrnl^−/−) and the effects of global knockout of Metrnl on blood lipid parameters under a normal chow diet. a Knockout strategy (left) and breeding strategy (right) for Metrnl^−/−. b Genotyping of the Metrnl^−/− mice. 1, Metrnl^wt/koElla-Cre; 2, Metrnl^wt/ko; 3, Metrnl^wt/wt; 4, Metrnl^ko/ko (Metrnl^−/−). c Metrnl levels in different tissues between Metrnl^−/− and wild-type mice (WT) (n = 6). ***P < 0.001 vs. WT. d Serum Metrnl concentration between Metrnl^−/− and WT mice (n = 6). e Body weights of Metrnl^−/− and WT mice fed a normal chow diet (n = 8). f The effects of global knockout of Metrnl on high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), total triglyceride (TG) and nonesterified fatty acid (NEFA) in circulation (n = 8)