Fig. 5.
The generation and identification of Metrnl liver-specific knockout mice (Liver-Metrnl−/−) and the effects of liver-specific knockout of Metrnl on blood lipid parameters. a Breeding strategy for Liver-Metrnl−/−. b Genotyping of Liver-Metrnl−/−. 1-3: Liver-Metrnl−/−; 4-6: Metrnlloxp/loxp used as control. c Metrnl levels were detected with real-time PCR in various tissues from Liver-Metrnl−/− and control mice (WT) (n = 6). ***P < 0.001 vs. WT. d Serum Metrnl levels were assayed with ELISA between Liver-Metrnl−/− and WT mice fed a normal chow diet (n = 6). e Serum Metrnl levels were assayed with ELISA between Liver-Metrnl−/− and WT mice fed a high-fat diet (HFD) (n = 3). f Body weights of Liver-Metrnl−/− and WT mice fed a normal chow diet (n = 9–10). g The effects of liver-specific knockout of Metrnl on blood high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), total triglyceride (TG) and nonesterified fatty acid (NEFA) under a normal chow diet (n = 9-10)