Skip to main content
. 2021 Mar 1;11:4920. doi: 10.1038/s41598-021-84187-9

Figure 2.

Figure 2

(a) Cell viability of Caco-2/HT29-MTX-E12 co-culture after 24 h exposure to increasing concentrations of chyme, measured using the WST-1 mitochondrial activity assay. Viability is given as a percentage of the control (% ± standard error of the mean (SEM); triplicates). A nonlinear curve was fitted through the points for clarity using GraphPad Prism. (b) Confocal image of Caco-2/HT29-MTX-E12 cells cultured in a transwell for 21 days (control). (c) Confocal image of Caco-2/HT29-MTX-E12 cells cultured in a transwell for 21 days and exposed to 12.5% chyme for 24 h. All exposures under static conditions. Cells were stained for tight junction protein ZO-1/TJP1 (red). Scale bar: 20 µm.