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. 2021 Mar 1;11:4849. doi: 10.1038/s41598-021-84351-1

Figure 6.

Figure 6

The influence of PARP1 or PARP2 and PARylation on the LigIIIα activity in the absence or presence of XRCC1. The ligation magnitude of the 5′[32P]labelled nicked NCP substrate under the action of LigIIIα was affected by PARP1 or PARP2 and PARylation in the absence or presence of XRCC1. The ligation magnitude was calculated as a percentage of the unreacted primer subtracted from 100%. The data come from experiments involving 20 nM NCP, 500 nM LigIIIα, 100 nM PARP1 or PARP2 and 100 μM NAD+. The data are presented as an average of at least three independent experiments and are shown as the mean values ± SD.