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. 2021 Mar 2;180:113122. doi: 10.1016/j.bios.2021.113122

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© 2021 Published by Elsevier B.V.

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Fig. 2 — Determination of the relative luciferase activity of the S1-ACE2 NanoBiT system under a variety of conditions. (A) Examination of pH stability of the S1-ACE2 biosensor looking at relative luminescence of cell lysates after lysis with NP40 lysis buffers ranging from pH 2-12. (B) Temperature stability of cell lysates containing the S1-ACE2 biosensor after a 30 min incubation at 0-80 °C. (C) Temperature stability of cell lysates containing the S1-ACE2 biosensor after 0-24 h incubations at 25, 30, or 37 °C. (D) S1-ACE2 biosensor activity measured in lysed cells (0-50000 Hek293T cells) 48 h post-transfection. (E) S1-ACE2 biosensor activity measured in live cells (0-50000 Hek293T cells) 48 h post-transfection.