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. 2021 Feb 16;12:625890. doi: 10.3389/fpls.2021.625890

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Copyright © 2021 Liu, Jin, Tan, Zheng, Gao, Zhang, Zhao, Ma and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alkaline stress caused less damage to the nitrogen content and the activity of enzymes involved in N metabolism in MdTyDc OE apples than in WT apples. (A) Leaf N content, (B) shoot N content, (C) root N content, and (D) total N content, (E) the activity of leaf NR, (F) root NR, (G) leaf NiR, (H) root NiR, (I) leaf GS, (J) root GS, (K) leaf GOGAT, and (L) root GOGAT. The data are presented as means ± SD (n = 3). Significant differences between WT and MdTyDc overexpression lines are indicated by different lowercase letters based on Tukey’s multi-range test (P < 0.05). WT, wild type. OE-2, MdTyDc overexpression line 2. OE-3, MdTyDc overexpression line 3. OE-5, MdTyDc overexpression line 5.