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. 2021 Feb 16;12:625890. doi: 10.3389/fpls.2021.625890

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Copyright © 2021 Liu, Jin, Tan, Zheng, Gao, Zhang, Zhao, Ma and Li.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Heatmap presenting expression levels of 6 ion-transport-related genes (MdSOS1, MdSOS2, MdSOS3, MdNHX1, MdHKT1, and MdAKT1), 15 N-absorption-related genes (MdAMT1.1, MdAMT1.2, MdAMT1.5, MdAMT1.6, MdAMT1.8, MdAMT2.1, MdAMT2.2, MdAMT3.1, MdAMT4.2, MdAMT4.3, MdNRT1.1, MdNRT1.2, MdNRT2.4, MdNRT2.5, and MdNRT2.7) and 7 C-metabolism-related genes (MdSPS1, MdSPS6, MdSUT2, MdNINV1, MdNINV2, MdCWINV1 and MdCWINV2) in (A) leaves and (B) roots of MdTyDc-overexpressing apple plants after 15 days under control and alkaline conditions. The data are presented as means ± SD (n = 3). Significant differences between WT and MdTyDc overexpression lines are indicated by different lowercase letters based on Tukey’s multi-range test (P < 0.05). WT, wild type. OE-2, MdTyDc overexpression line 2. OE-3, MdTyDc overexpression line 3. OE-5, MdTyDc overexpression line 5.