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. 2021 Feb 16;12:636154. doi: 10.3389/fphar.2021.636154

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Copyright © 2021 Xiong, Deng, Wang, Shao, Zhou, Zou and Zhuang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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I-BET151 abrogates the TGF-β/Smad3 signaling pathway in kidneys of HN rats. (A) Protein was extracted from kidneys and subjected to measurement of TGF-β1 by ELISA as indicated. (B) Expression levels of p-Smad3 were quantified by densitometry and normalized to Smad3. (C) The kidney tissue lysates were subjected to immunoblot analysis with specific antibodies against p-Smad3, Smad3, p- ERK1/2, ERK1/2, or GAPDH. (D) Expression levels of P-ERK1/2 were quantified by densitometry and normalized to ERK1/2. (E) Photomicrographs (original magnification, ×400) illustrate immunohistochemical staining for p-ERK1/2 in kidney tissues. (F) p-ERK1/2 staining graphic presentation of quantitative data. Data are represented as the mean ± SEM. *p < 0.05; **p < 0.01.