Figure 1.

Immunocytochemical characterization of human and rat cells used in the biofabrication of heterocellular spheroids cultured in flat well plates

(A–G) (A) Scheme of the neurovascular units and (B–G) CLSFM micrographs of (B) hCMEC/D3 endothelial cells cultured on collagen-coated glass coverslips and their specific VE-cadherin (red) and CLDN5 (green) staining, (C) GFAP-positive primary hAs, (D) NG2-positive hBVPs (green), (E) βIII-tubulin-positive primary rat neurons (red), (F) Iba-1/AIF-1-positive primary rat microglia, and (G) iNOS-positive primary rat microglia. Cell nuclei in (B–F) are stained with 4′,6-diamidino-2-phenylindole (DAPI, blue).