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Immunocytochemical characterization of the biofabricated 5-cell spheroids
Representative (A–G) CLSFM and (H–L) LSFM micrographs. Spheroids show the expression of characteristic markers of (A, B, H, I, and J) hCMEC/D3 endothelial cells, (C, K, and L) primary hAs, (D) hBVPs, (E and F) primary rat neuron, and (G, I, and J) primary rat microglia. Cell nuclei in A, C, E, H, J, and K are stained with 4′,6-diamidino-2-phenylindole (DAPI, blue).
