Table 1.
Genetics and characteristics of Zika virus (ZIKV) and dengue virus (DENV) neutralizing monoclonal antibodies.
| Antibody | Heavy chain | VH% SHM | Light chain | HCDR3 length | VDJ junction | Epitope | Neutralization potency | Cross neutralization | References |
|---|---|---|---|---|---|---|---|---|---|
| ZIKV-117 | VH3-30 | VK3-15 | 12 | Quaternary | **** | ZIKV specific | (45) | ||
| 5J7 | VH1-69 | VK1-39 | 14 | CARDKELLFSRAFDIW | Quaternary | **** | DENV-3 specific | (22, 47) | |
| MZ4 | VH4-59 | 5.5 | VL1-44 | 14 | CAGLDRYSWNEGGDHW | DI-DIII linker | **** | ZIKV, DENV2-3 | (20) |
| Z004 | VH3-23 | VK1-5 | 15 | CAKDRGPRGVGELFDSW | DIII lateral ridge | **** | ZIKV, DENV-1 | (34) | |
| Z006 | VH3-23 | VK1-5 | 13 | CVRDRSNGWSSINLW | DIII lateral ridge | **** | ZIKV, DENV-1 | (34) | |
| SMZAb5 | VH3-23 | VK1-5 | 15 | CAKDRSTRGFGELLNYW | DIII lateral ridge | **** | ZIKV, DENV-1 | (33) | |
| ZIKV-116 | VH3-23 | 6.5 | VK1-5 | 15 | CAKDRLSRGVGELYDSW | DIII lateral ridge | *** | ZIKV, DENV-1 | (36, 45) |
| 1C11 | VH3-23 | 3.5 | VK1-5 | 14 | CAKDRIVLGLELFDSW | DIII lateral ridge | ** | ZIKV, DENV-1 | (35) |
| ZKA190 | VH3-30 | 2.7 | VK3-20 | 23 | CAKSGTQYYDTTGYEYRGLEYFGYW | DIII lateral ridge | *** | ZIKV specific | (42) |
| EDE1-C8 | VH3-64D | 6.9 | VK3-11 | 15 | CVGGYSNFYYYYTMDVW | E dimer | *** | ZIKV, DENV1-4 | (25, 27) |
| EDE1-C10 | VH1-3 | 2.8 | VL2-14 | 21 | CARDKVDDYGDYWFPTLWYFDYW | E dimer | **** | ZIKV, DENV1-4 | (25, 27) |
| EDE2-A11 | VH3-74 | 8.7 | VL2-23 | 26 | CVRDGVRFYYDSTGYYPDSFFKYGMDVW | E dimer | ** | ZIKV, DENV1-4 | (25, 27) |
| 2D22 | VH1-69 | VL1-47 | 9 | CARRPQSIFDW | E dimer | ** (DENV-2) | DENV-2 specific | (22) | |
| MZ20 | VH3-11 | 10.4 | VK1-33 | 10 | CVRAGGARIENW | DII bc loop | ** (ZIKV) | ZIKV, DENV1-4, JEV | (20) |
| MZ54 | VH3-11 | 10.4 | VK1-33 | 10 | CVCAGGGRTDYW | Fusion loop | ** | ZIKV, DENV1-4, WNV | (20) |
| MZ56 | VH3-64 | 4.9 | VL2-11 | 17 | CARGWYYYDSRAYWYFDLW | Fusion loop | ** | ZIKV, DENV1-4, WNV | (20) |
| Z3L1 | VH3-30 | VL1-51 | 10 | CARDHLGWSSIW | DI, DI-DII hinge | ** | ZIKV specific | (21) | |
| 1F4 | VH3-33 | VL1-36 | 18 | CARDKNPGTKPYYHYGMDVW | DI, DI-DII hinge | *** | DENV-1 specific | (22, 23) |
VH % SHM: % somatic hyper mutation in the heavy chain variable gene. VH % SHM and VDJ junction characteristics were indicated whenever available. Neutralization potency is based on IC50 against ZIKV unless otherwise indicated. IC50 (ng/ml) are indicated as follows: **** (<10); *** (10–50); ** (50–500); * (>500). DIII lateral ridge-directed antibodies sharing heavy and light chain V gene usage are underlined.