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. 2021 Feb 16;12:632503. doi: 10.3389/fpls.2021.632503

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Copyright © 2021 Hu, Lung, Ye and Chye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GUS activity assays of transgenic Arabidopsis AtACBP3pro::GUS plants following wounding. (A) Four-week-old AtACBP3pro::GUS transgenic plants were wounded with a pair of forceps and harvested at 1, 2, 3, and 4 h post wounding (hpw) for quantitative GUS activity measurements. Vector (pBI101.3)-transformed plants were analysed at 1 hpw and served as a negative control, while unwounded AtACBP3pro::GUS plants were used as a baseline control. “^**” indicates statistically significant difference (P < 0.01, n = 3 by Student's t-test) in comparison to unwounded samples collected at the same time point. Error bars represent standard deviations. (B) A representation of histochemical staining of wounded 4-week-old leaves from AtACBP3pro::GUS and vector (pBI101.3)-transformed (VC) Arabidopsis. These experiments were repeated twice with consistent results. Scale bars = 0.8 cm.