Figure 4.

Comparison of the temporal profiles of cortical microglial heterogeneity of Wt and gp120 Tg mice

(A) tSNE plots of Wt and Tg cortical microglia at different ages. The cortical microglial subtypes of the Tg mice at two, four, and eight months increased, compared to the Wt counterparts at the corresponding ages; this was particularly clear at 4 months. The signature genes expressed in the individual clusters were indicated in the pink boxes.

(B) Heatmap showed the expression of feature genes of IFLAM-M, myelination/demyelination-related microglia (MYE-M), INF-M, and PLF-M subtypes. MYE-M (black rectangle) were only observed in the four-month-old Tg cortices, while INF-M (blue rectangle) gradually increased with age progression in Tg cortices. The columns represent the subsets of all microglia from each data set.