Figure 5.
Verification of MYE-M in the Wt and Tg spinal and cortical tissue of four-month-old mice
(A) Feature plots showing the signature genes of MYE-M in the four-month-old Tg cortex (4mTgBr).
(B) Venn diagram showed that 13% of the up-regulated genes in MYE-M were expressed in DAM (disease-associated microglia) identified in Alzheimer disease (see also Table S1).
(C) RNAscope in situ hybridization (ISH) demonstrated the expression of Cst7 mRNAs (white) in the Wt and Tg cortical microglia (red) (labeled by Iba1 immunostaining).
(D) Population of Cst7+Iba1+ microglia in the Wt and Tg cortex and spinal cord.
(E) Fluorescent immunostaining revealed the expression of Lgals3 (red) protein in the Wt and Tg cortical microglia (green) (labeled by Iba1 immunostaining).
(F) The population of Lgals3+Iba1+ microglia in the Wt and Tg spinal cord and cortex. ∗∗(orange): p value<0.01 4mWtSp vs 4mTgSp. ∗∗: p value <0.01 4mWtBr vs 4mTgBr. ††: p < 0.05 4mTgSp vs 4mTgBr. n = 3. Scale bar: 10 μm. Data are represented as mean ± standard error of the mean (SEM).