Verification of INF-M in the Wt and Tg spinal and cortical tissue of four-month-old mice
(A) ISH demonstrated the expression of Irf7mRNA (green) (one of INF-M marker genes) in the cortical microglia (red) (labeled by Iba1 immunostaining).
(B) Population of Irf7+Iba1+ microglia in the Wt and Tg spinal cord and cortical tissue of four-month-old. Scale bar: 10 μm. ∗∗(orange): p value<0.01 4mWtSp vs 4mTgSp. ∗∗: p value <0.01 4mWtBr vs 4mTgBr. ††: p < 0.05 4mTgSp vs 4mTgBr. n = 3. Scale bar: 10 μm. Data are represented as mean ± standard error of the mean (SEM).