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Cell viability of 3D HepG2 spheroids evaluated with WST-1 assay. 72 h after exposure to ibuprofen +/- EtOH. N=3, *p<0.05, **p<0.01. Histological changes in 3D cultured HepG2 after exposure to ibuprofen with or without EtOH. Red arrows indicate cell nucleus accumulation.
