Fig. 3.

Late passage astrocytes show decreased wound healing and phagocytosis ability. (A) Representative images of astrocytes were obtained through the wound-healing assay. Blue lines represent the initial scratch-wound area. Red lines indicate wound closure at each time-point. Images and data gathered using IncuCyte (Essen Bioscience). (B) Cell migration every 12 h into the wound area, represented by wound width (μm). (C) Astrocytes were examined for its phagocytosis ability using E. coli BioParticles. (D) The graphs represent the total red object area per well (μm²/well) and the total red object integrated intensity (RCU×μm²/image) every 30 min for 12 h. Lines show the mean ± SEM (n=3). ***indicates p<0.001 vs. vehicle group.