Table 2.

Sequences of primers used for quantitative real-time PCR.

Gene	Full name	Sequence	Amplicon size (bp)	Ta oC	References
IgA	Immunoglobulin A	GTCACCGTCACCTGGACACCA	192	64	(Lammers et al., 2010)
		ACCGATGGTCTCCTTCACATC
IgG	Immunoglobulin G	ATCACGTCAAGGGATGCCCG	118	60	(Zhao et al., 2013)
		ACCAGGCACCTCAGTTTGG
IgM	Immunoglobulin M	GCATCAGCGTCACCGAAAGC	98	60	(Zhao et al., 2013)
		TCCGCACTCCATCCTCTTGC
CLDN1	Claudin 1	CTTCATCATTGCAGGTCTGTCAG	103	60	(Zanu et al., 2020)
		AAATCTGGTGTTAACGGGTGTG
CLDN5	Claudin 5	GCAGGTCGCCAGAGATACAG	162	61	(Zanu et al., 2020)
		CCACGAAGCCTCTCATAGCC
OCLD	Occludin	ACGGCAGCACCTACCTCAA	123	60	(Du et al., 2016)
		GGGCGAAGAAGCAGATGAG
JAM2	Junctional adhesion 2	AGACAGGAACAGGCAGTGCTAG	135	60	(Zanu et al., 2020)
		ATCCAATCCCATTTGAGGCTAC
TJP1	Tight junction protein 1	GGATGTTTATTTGGGCGGC	187	60	This study
		GTCACCGTGTGTTGTTCCCAT
TLR5	Toll-like receptor 5	TGTGGGAGAGAGGTTTATGTTTGG	169	60	(Yang et al., 2015)
		CTGAGAGAGAGGTGAGACAATAGG
TLR21	Toll-like receptor 21	AGTTGTGTCCTGTGCTGAGAG	130	60	(Wang et al., 2015)
		AGCAGGTTGTGTTCCACTGTC
IL2	Interleukin 2	TCTGGGACCACTGTATGCTCT	256	60	(Liu et al., 2018)
		ACACCAGTGGGAAACAGTATCA
IL6	Interleukin 6	CAAGGTGACGGAGGAGGAC	254	60	(Hong et al., 2012)
		TGGCGAGGAGGGATTTCT
IL7	Interleukin 7	GGTTCTGCCACTTCTCCTTG	160	60	(Khan et al., 2020)
		CTTGCAGCATCTGTCACGATA
IL12-β	Interleukin 12β	TGGGCAAATGATACGGTGAA	83	60	This study
		CAGAGTAGTTCTTTGCCTCACATTTT
IL18	Interleukin 18	TGTGTGTGCAGTACGGCTTAG	79	60	(Forder et al., 2012)
		CTTACAAAAGGCATCGCATTC
IFN-γ	Interferon gamma	AGCTGACGGTGGTGGACCTATTATT	259	60	(Liu et al., 2018)
		R-GGCTTTGCGCTGGATTC
TGF-4β	Transforming growth factor-4β	AGGATCTGCAGTGGAAGTGGAT	137	60	This study
		CCCCGGGTTGTGTTGGT
HPRT1	Hypoxanthine phosphoribosyl-transferase 1	ACTGGCTGCTTCTTGTG	245	63	(Yang et al., 2013)
		GGTTGGGTTGTGCTGTT
TBP	TATA-Box binding protein	TAGCCCGATGATGCCGTAT	147	62	(Li et al., 2005)
		GTTCCCTGTGTCGCTTGC