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Primers for PCR amplifications to detect ALV.
| Name | Sequence (5′-3′) | Annealing temperature (°C) | Expected size (bp) |
|---|---|---|---|
| ALV-A-F | GGATGAGGTGACTAAGAAAG | 50 | 692 |
| ALV-A-R | AGAGAAAGAGGGGTGTCTAAGGAG | ||
| ALV-B-F | GGATGAGGTGACTAAGAAAG | 50 | 847 |
| ALV-B-R | ATGGACCAATTCTGACTCATT | ||
| ALV-C-F | GGATGAGGTGACTAAGAAAG | 50 | 860 |
| ALV-C-R | GAGGCCAGTACCTCCCACG | ||
| ALV-D-F | GGATGAGGTGACTAAGAAAG | 50 | 797 |
| ALV-D-R | ATCCATACGCACCACAGTATTCG | ||
| ALV-J-F | GGATGAGGTGACTAAGAAAG | 56 | 545 |
| ALV-J-R | CGAACCAAAGGTAACACACG |
Abbreviation: ALV, avian leukosis virus.
