Figure 5.

EGFR accumulation in UVRAG-deficient muscle. (A) Immunoblots (left) and quantification (right) of epidermal growth factor receptor (EGFR), phospho- and total-Akt, and phospho- and total-Stst3 from quadriceps of the 6-month-old mice (n = 5). (B) Immunoblots of UVRAG and EGFR in the cytosolic (Cy) and mitochondrial (Mt) fractions of C2C12 myotubes transfected with scramble (Scr) or UVRAG siRNA (siUV) (n = 3). GAPDH and VDAC are representative cytosolic and mitochondrial control proteins respectively. WC: whole cell lysate. Data shown are mean ± S.E.M. ∗∗p < 0.01 and ∗∗∗p < 0.001 using Student's t test.