Effects of stimulation frequency on the complete post-LFBS block induced by LFBS at the minimal intensity and duration. A longer stimulation duration is required for higher frequencies to induce a complete post-LFBS block (a). However, complete block duration (b) and recovery period (c) do not change with stimulation frequency (p > 0.05, un-paired t-test). The nerve block can occur at intensities of 1–3 T (3–9 mA) at different frequencies. N = 6 for 100 Hz, N = 9 for 500 Hz, and N = 14 for 1 kHz. * indicates significant difference (p < 0.05, un-paired t-test).