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Estimation of apparent binding affinity to CT26 cells by free PD-L1 Apt (A) or Apt-HJ (B). CT26 cells were incubated with FAM-labeled PD-L1 aptamers or Apt-HJ of various concentrations for 30 min, washed thrice in PBS, and analyzed by flow cytometry. Duplicate samples (n = 2) were used for each concentration in the experiment. The mean of the two fluorescence measurements was used in the chart.
