Figure 5.

17-AAG inhibits HAdV-5 transcription and DNA replication early after infection. A549 cells were infected with HAdV-5. At the indicated times 17-AAG was added to a final 0.25 μM concentration (dotted line) or left without the inhibitor (solid line) and culture was prolonged to 24 h. 24 h after infection DNA and RNA was analyzed by q-PCR with primers specific for E1A, DBP, and hexon transcripts. DNA was analyzed using hexon-specific primers. The presented results are means and SD values from three experiments, normalized to results obtained for GAPDH.