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Hsp90 inhibition does not increase degradation of the mature E1A. (A) HEK293 cells were cultured for 24 h with the indicated concentrations of 17-AAG. (B) HEK293 cells were incubated with 500 nM 17-AAG, or with 500 nM 17-AAG and 100 µg/mL cycloheximide (CHX) for 9 and 24 h. Control cells were cultured without the inhibitors. E1A and GAPDH in the extracts were detected by western blot. Representative western blot, one of two performed is shown.
