Figure 1.

Fluorescence signal is detectable at the tumor using an anti-CEA nanobody conjugated to IRDye800CW using clinically available FDA-cleared fluorescence imaging devices. Images were acquired after placing the animal within the minimally-invasive simulation dome (A) and switching between the bright light and the fluorescence mode (B). Representative images from fluorescence imaging using the Stryker Aim laparoscopic camera (Kalamazoo, MI, USA) and the corresponding bright light image (C,D). Tumors are approximately 7 mm–1 cm at the time of imaging.