Figure 2.
YAP1 expression and chromosome 11 status in parathyroid adenomas. (a) YAP1 gene expression levels in parathyroid adenomas (Pads) with a normal haplotype for chromosome 11 (PAds WT) or PAds harboring loss of heterozygosity of the chromosome 11 (PAds LOH); YAP1 mRNA levels were relatively quantified on that detected in three normal parathyroid glands and log2-transformed. (b) Correlation between YAP1 and MEN1 gene expression levels in PAds harboring the chr.11 LOH (r2 = 0.561, p = 0.032), and (c) in PAds with no loss of chr.11 (WT). (d) Transient downregulation of MEN1 by siRNA in PAds-derived cells (n = 4) was verified by qPCR relative to cultures treated with a mock siRNA (Control) or left untreated (NT); * p = 0.006; ** p = 0.011 by one-way ANOVA. (e) YAP1 mRNA expression was analyzed by qPCR after transient transfection with a MEN1 or a control siRNA in primary PAd cultures and expressed relative to untreated (NT) samples; * p = 0.036 by one-way ANOVA. (f) Transient downregulation of the YAP1 or a control transcript by siRNA in HEK293A cells (n = 3) was verified by qPCR relative to untreated samples (NT); *, p = 0.008 by one-way ANOVA. (g) MEN1 expression was analyzed by qPCR in HEK293A cultures after transient transfection with a YAP1 or a control siRNA (n = 3) and expressed relative to untreated samples (NT); * p = 0.021 by one-way ANOVA.