Skip to main content
. 2021 Feb 18;13(4):857. doi: 10.3390/cancers13040857

Figure 2.

Figure 2

Ad.5-TCTV inhibits cancer cell proliferation to a similar extent as Ad.5-CTV. (A–D) The indicated cell types (5000 cells), (A) human breast cancer (MDA-MB-231 and SUM-159); (B) human prostate cancer (DU-145 and PC-3); (C) primary normal human breast epithelial (HMEC); and (D) normal immortal prostate epithelial (RWPE-1) cells were plated in 96-well plates and infected with either Ad.5-E1A (25 pfu), Ad.5-CTV (25 pfu), or Ad.5-TCTV (25 pfu) for 72 h. These cells were then treated with 20 μL of MTT in phosphate-buffered saline (PBS) and incubated further for 4 h. Media was then removed and formazan crystals were dissolved using dimethyl sulphoxide (DMSO) (100 μL). A microplate reader was used to measure the absorbance at 550 nm and the results are presented as percent proliferation with the comparison of cells treated with vehicle. Points, mean of triplicate experiments; bars, S.E. * p < 0.05 vs. control.