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The TCTV does not induce apoptosis in normal breast and prostate epithelial cells. Normal primary breast epithelial (HMEC) and normal immortal prostate epithelial (RWPE-1) cells were cultured in 8-well chamber slides and treated with 25 pfu of Ad.5-E1A, Ad.5-CTV, or Ad.5-TCTV for 72 h. Cells were fixed and TUNEL assays were performed. Data presented as TUNEL positive cells in a defined microscopic field as compared with the un-treated control cells. Scale bar: 200 μM.
