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. 2021 Feb 19;22(4):2043. doi: 10.3390/ijms22042043

Figure 6.

Figure 6

Epithelial apoptosis in HT-29/B6-GR/MR cell monolayers in monoculture or co-culture conditions (with M1-macrophage-like THP-1 cells on the basal side) after Campylobacter concisus infection. (A) The apoptotic cells in HT-29/B6-GR/MR cell monolayers were stained green by TUNEL assay kit and nuclei were stained blue by DAPI. (B) Percentage of apoptotic cells (TUNEL-positive) cells, quantified in five to eight randomly picked regions of the cell monolayers (n = 3 in independent experiments, ns = not significant, ** p < 0.01, *** p < 0.001).