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. 2021 Feb 17;9(2):166. doi: 10.3390/vaccines9020166

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Mean EIV-specific serum neutralizing antibody titers determined by virus neutralization (VN) assay. Serum samples were collected from groups 1 (G1) and 2 (G2) after 6 months of immunization (V1—day 0) with either Calvenza-03 EIV/EHV^® (group 1; G1: black circles) or Fluvac Innovator^® (group 2; G2: black squares) and after 10 weeks (V2—10 weeks) of booster immunization with Calvenza-03 EIV/EHV^® for horses of both groups (G1: upright black triangles; G2: downright black triangles), and VN assay was performed. The central line in the scattered dot plot indicates the mean of the data with standard error of mean (error bars). * A significant increase (2-way repeated measures ANOVA with Tukey’s multiple comparisons post-test; p < 0.05) of antibody titer for group 1 was seen when compared to group 2. NS—not significant; vacci.—vaccination.