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. 2021 Feb 18;22(4):2025. doi: 10.3390/ijms22042025

Figure 4.

Figure 4

Decreased Myeloid-derived suppressor cells (MDSCs) in CCL17 transgenic (TG) mice. MDSCs are defined as CD11b+Gr-1+ in mice. CCR4 is expressed by Tregs and Th2 cells. Expression levels of CCR2, CCL5, and CXCL17, which are all related to MDSCs, and IL-4, which is produced by Th2 cells, were evaluated by quantitative reverse transcription-PCR in skin tumors from wild-type (WT) and CCL17 TG mice. (A) Representative flowcytometric images showing the frequency of CCR4+ cells, Gr-1+ cells−, and CD11b+ cells. (B) The frequency of CCR4+ cells was significantly higher in CCL17 TG mice, while the frequency of CD11b+Gr-1+ cells was significantly lower compared with that of WT mice, respectively. (C) There is a negative correlation between the frequency of CCR4+ cells and that of CD11b+Gr-1+ cells. (D) Expression levels of CCR2, CCL5, and CXCL17 mRNA were significantly downregulated in CCL17 TG mice, while IL-4 mRNA levels were significantly upregulated in CCL17 TG mice compared with those in WT mice. * p < 0.05 WT versus CCL17 TG mice.