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. 2021 Feb 19;26(4):1084. doi: 10.3390/molecules26041084

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Ultra-high-performance liquid chromatography-ultraviolet/photo-diode array (UHPLC-UV/PDA) chromatogram of commercial standards recorded at 340 nm: 5, isoorientin; 6, orientin; 10, vitexin; and 11, isovitexin. (B) Typical chromatogram of OPL crude and hydrolyzed extracts at 340 nm. Peak identified: 1, luteolin-6,8-di-C-hexose (Isomer 1); 2, apigenin-6,8-di-C-hexose; 3, luteolin-6,8-di-C-hexose (Isomer 2); 4, apigenin-6-C-pentose-8-C-hexose (Isomer 1); 5, isoorientin; 6, orientin; 7, luteolin-6-C-hexose-8-C-deoxyhexose (Isomer 1); 8, apigenin-6-C-pentose-8-C-hexose (Isomer 2); 9, luteolin-6-C-hexose-8-C-deoxyhexose (Isomer 2); 10, vitexin; 11, isovitexin; and 12, apigenin-6-C-hexose-8-C-deoxyhexose.