Figure 3.

An overview of the in vitro-in silico modeling approach for a conservative estimate of glucose response curves after the intake of a specified serving size of a dish or a ready-to-eat food product. In general, this approach can be divided into three steps: (1) pretreatments of a specified size of dish or food product to be digested in vitro, which may include intensive mixing and grinding by a household blender to destroy the food structures, thus maximumly facilitating the release of glucose during digestion; (2) measurement of the maximum amount of convertible glucose released from the food tested under standardized in vitro digestive conditions; (3) application of the in silico glucose–insulin regulation model to obtain a conservative estimate of the blood glucose rise with the measured maximum in vitro convertible glucose as input data. Assuming this convertible amount will be processed as one glucose sample in the digestion tank; it presents a maximum possible blood glucose rise. This is viewed as a conservative indicator for a consumer.