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. 2021 Feb 19;11(2):349. doi: 10.3390/diagnostics11020349

Table 6.

Results when isolates were tested on an incorrect panel or using an incorrect algorithm, i.e., inoculation of Gram-negative species on Gram-positive panels or vice versa, or of Gram-positive species incorrectly assigned as “Streptococcus” or “Micrococcus” based on faulty catalase or microscopy results. The expected result for this type of incorrect panel use is “identification not possible”. For Gram-negative species inoculated on Gram-positive panel, we assumed Micrococcus interpretation for the results in this table, as most of them would be catalase positive. Note that all results other than “identification not possible” are wrong; detailed results for each species can be found in Supplementary Table S3. Incorrect identifications with high probability are listed in Table 5. N = total number of isolates tested.

Species. N Panel/Algorithm Used Number “Identification not Possible” (%) Number Low Probability Identification (%) Number High Probability Identification (%)
Gram-negative species tested on Gram-positive panels and vice versa
Enterobacterales 41 Gram-positive 27 9 5
Non-fermenters 23 10 6 7
Total Gram-negatives on Gram-positive panel 64 37 (57.8%) 15 (23.4%) 12 (18.8%)
Staphylococcus species 16 Gram-negative 1 11 4
Streptococcus species 53 15 12 26
Gram-positive bacilli (Bacillus & Corynebacterium species) 11 2 4 5
Total Gram-positive on Gram-negative panel 80 18 (22.5%) 27 (33.8%) 35 (43.8%)
Staphylococcus/Micrococcus species using Streptococcus species algorithm and vice versa
Staphylococcus/Micrococcus species and Gram-positive rods 60 Streptococcus algorithm 35 11 14
Streptococcus/Enterococcus species 95 Micrococcus algorithm 27 24 44
Total Gram-positive with incorrect algorithm 155 62 (40.0%) 35 (22.6%) 58 (37.4%)
Total tested on incorrect panel/incorrect algorithm 299 117 (39.1%) 77 (25.8%) 105 (35.1%)