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. 2021 Feb 18;13(2):315. doi: 10.3390/v13020315

Figure 1.

Figure 1

Increased ribonuclease L (RNase L) activity in cells with reduced ATP-binding cassette E1 (ABCE1) protein levels. (A) Knockdown of ABCE1 levels using short hairpin RNA (shRNA) in HT1080 WT cells or CRISPR/Cas9 RNase L KO cells was verified in cell lysates by immunoblotting using specific antibodies. (B) WT, ABCE1 KD, RNase L KO, and ABCE1 KD/RNase L KO cells were transfected with 2-5A (10 µM) or 2 µg/mL of poly I:C for 6 h, and the RNase L-mediated cleavage of rRNA (arrows) was analyzed on RNA chips using Agilent Bioanalyzer 2100. (C) The cleavage of rRNA was quantitated and normalized to mock-treated samples. Data are representative of at least three independent experiments and expressed as means ± SD; WT: wild-type; ** p < 0.01; *** p < 0.001.