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. 2021 Feb 18;10(2):435. doi: 10.3390/cells10020435

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunohistochemical analysis of inducible COX (COX-2; red fluorescence) in liver (A), kidney (B), aorta (C), and heart (D) from untreated (saline solution) Aqp9^+/+ or Aqp9^−/− mice (WT CTRL and KO CTRL, respectively) or LPS-treated (40 mg/kg) WT or KO mice (WT+LPS and KO+LPS, respectively) (four animals/group), 6 h after the i.p. injection. The green fluorescence seen in aortas corresponds to elastin autofluorescence. Cell nuclei were stained by DAPI (blue fluorescence). C(−), negative controls (no primary antibody).