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. 2021 Feb 18;22(4):2037. doi: 10.3390/ijms22042037

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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ABRACL expression affects cancer cell migration. (A–D) Western analysis on cell lysates using antibodies against ABRACL and α-tubulin (loading control), and quantitative results of Transwell migration assays. The migration index was calculated by normalizing the number of migratory cells in each test sample to that of the control; representative micrographs and means ± SD from at least 3 independent experiments are shown; *, p < 0.05. (A) MDA-MB-231 cells transiently transfected with the control pCMV-HA (−) or pCMV-HA-ABRACL (+) vector. (B) Stable clones of HCT116 infected with the control luciferase-targeting (ShLuc) or ABRACL-targeting (Sh295 and Sh484) shRNA-expressing lentivirus. (C) Stable control (ShLuc) and ABRACL-knockdown (Sh484) HCT116 clones transfected with the control HA- (−) or HA-ABRACL (+)-expressing vector. (D) Parental and independent ABRACL-knockout HCT116 clones generated by CRISPR/Cas9-mediated gene editing using two different ABRACL-targeting sequences (G2 and G3).