Skip to main content
. 2021 Feb 18;22(4):2037. doi: 10.3390/ijms22042037

Figure 2.

Figure 2

ABRACL modulates elicited cell migration. (A) Western analysis on cell lysates from parental and independent CRISPR-mediated ABRACL-knockout MDA-MB-231 stable clones (G1-3, G2-2, and G2-25). (B–C), Wound healing assay. Shown on the left are representative micrographs of the wound regions; parallel black dashed lines indicate the wound edge at 0 h. Quantitative data are shown on the right; the area of the wound region was measured using ImageJ software, and the extent of wound closure relative to that of the parental cells was calculated. Mean ± SD from at least 3 independent experiments are shown; *, p < 0.05. (B) Cells assayed in a standard medium. (C) EGF-stimulated wound healing. Cells were incubated in a serum-free medium for 16 h before an in vitro wound was made at time 0 and further incubated in the absence or presence of 10 ng/mL EGF; images were taken at 0 and 6 h to determine the wound closure efficiency.