Figure 7.

ABRACL can affect actin dynamics in vitro. (A) In vitro actin polymerization assay. Pyrene G-actin was incubated with test buffer alone or with indicated concentrations of purified recombinant ABRACL. The intensity of emitted fluorescence in each mixture was monitored using the TECAN-infinite reader. Shown are normalized fluorescence intensities in arbitrary units (a.u.) in relation to the incubation time after initiating polymerization by 1X actin polymerization buffer at time 0. (B–C), In vitro actin depolymerization assay. Preformed pyrene F-actin was incubated with purified ABRACL alone at indicated concentrations (B) or with cofilin (CFL) in the absence or presence of ABRACL (ABR) at indicated concentrations (C), and the intensity of emitted fluorescence was monitored. Shown are normalized fluorescence in relation to the incubation time after adding test proteins at time 0.