Figure 1.

Optimization of G-quadruplexes (G4s) detection. Samples were treated by (A) Turbo-DNAse or (B) RNase for 30 min at 37 °C, and after immunofluorescence (Anti-DNA G-quadruplex (G4) Antibody, clone 1H6 was used), the presence/absence of G4s structures (red) was analyzed inside interphase nuclei (blue). In both panels, cells were additionally visualized in transmission light (the bright field microscopy). Scale bars indicate 20 µm.