Skip to main content
. 2021 Feb 18;7(2):149. doi: 10.3390/jof7020149

Figure 4.

Figure 4

Heterologous complementation at the genomic locus. (A): Schematic drawing of the U. maydis (Um) mat1 gene indicating the position of the PAM site. Donor DNA contained the ORF for Mat1 from U. hordei (Uh) and 500 bp upstream and downstream flanking sequence of the U. maydis ORF. Further details are depicted in Figure S2 and Table S3. (B): Thin layer chromatography of glycolipids from MB215 (wt), ∆rua1, ∆rua1 ∆mat1 and ∆rua1 Uhmat1. MEL variants (A,C,D) can be separated according to their acetylation patterns.