Table 2.

Sample characteristics obtained with HPLC analysis.

Biogenic Amines	Linear Range (μg/mL)	R2	LOD (μg/mL)	LOQ (μg/mL)	Intra-Day Precision (%RSD) ( n = 5)	Inter-Day Precision (%RSD) ( n = 5)	Recovery (Young Leaves Samples) (% mean ± SD, n = 3)
							1 μg/mL	5 μg/mL	10 μg/mL
MET	0.10–10	0.9999	0.015	0.05	0.98	2.54	89.1 ± 4.9	91.7 ± 1.3	86.5 ± 1.1
TRP	0.10–10	0.9990	0.030	0.10	0.81	1.53	79.3 ± 4.6	92.1 ± 1.2	91.7 ± 1.0
PEA	0.25–10	0.9997	0.075	0.25	1.01	1.73	92.6 ± 5.3	94.3 ± 1.6	90.5 ± 1.2
PUT	0.10–50	0.9996	0.015	0.05	2.69	4.38	88.2 ± 5.4	90.6 ± 1.6	88.6 ± 1.2
CAD	0.10–10	0.9998	0.030	0.10	0.93	1.67	86.3 ± 4.3	93.6 ± 1.6	95.5 ± 1.1
HIS	0.10–10	0.9993	0.030	0.10	1.27	2.39	89.5 ± 4.7	91.2 ± 1.2	87.9 ± 1.1
5-HT	0.25–10	0.9989	0.075	0.25	1.33	2.06	110.3 ± 5.1	88.1 ± 2.0	87.7 ± 1.7
TYR	0.25–10	0.9997	0.075	0.25	0.66	0.91	86.5 ± 4.1	88.2 ± 1.1	89.4 ± 1.3
SPD	0.10–10	0.9997	0.030	0.10	1.42	2.82	89.7 ± 5.7	92.6 ± 1.9	90.4 ± 1.4
DA	0.25–10	0.9989	0.075	0.25	1.09	2.43	93.1 ± 4.8	94.1 ± 2.5	88.5 ± 1.7
SPM	0.10–10	0.9996	0.030	0.10	2.07	3.31	96.5 ± 4.4	94.2 ± 1.4	90.1 ± 1.1

R²: square of regression coefficient; LOD: limit of detection; LOQ: limit of quantification; RSD: relative standard deviation.