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The CRISPR-Cas system including tracrRNA (trans-activating crRNA), Cas9 protein and crRNA (CRISPR RNA) delivered by engineered phages against antibiotic-resistant bacteria. (A) Phage-delivered CRISPR-Cas9 could lead to cell death and plasmid loss by targeting genes on the (i) chromosome (aph-3/eae/nuc) and (ii) plasmid (mecA/blaNDM-1/blaSHV-18), respectively [16,17,24]. (B) Phage-delivered CRISPR-Cas3 targets specific DNA sequences (ndm-1/ctx-M-15) in plasmid [23].
