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. 2021 Feb 18;22(4):2003. doi: 10.3390/ijms22042003

Figure 3.

Figure 3

nc886 suppresses NF-κB and AP-1, two PKR downstream pathways. (A) Luciferase assays. Poly(I:C)-HMW was used. Poly(I:C) or an IRF3(5D)-expressing plasmid was co-transfected with luciferase plasmids, followed by cell harvest at 24 h. All the other descriptions are the same as Figure 1D. (B) qRT-PCR measurement of indicated NF-κB target genes, at 3 h after transfection of Poly(I:C). (C) Luciferase assays as described in Figure 1D. (D) Western blot of indicated proteins, as described in Figure 2B. Cells were harvest at 24 h after transfection of anti-oligos. One, two, and three asterisks designate a p-value less than 0.05, 0.01, and 0.001, respectively.