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. 2021 Feb 18;22(4):2003. doi: 10.3390/ijms22042003

Figure 5.

Figure 5

nc886 attenuates inflammatory responses in macrophages triggered by LPS. (A) Northern hybridization of nc886 and 5S rRNA as a loading control. (B) Luciferase assays. Transfection of luciferase plasmids at 0 h, treatment of LPS (10 ng/mL) at 6 h, and cell harvest and luciferase assays at 24 h. All other descriptions are the same as Figure 1D. (C) qRT-PCR of indicated genes at 6 h after treatment with LPS (100 ng/mL). (D) ELISA to measure secreted IL6 and NO, at 24 h after treatment with LPS (100 ng/mL). One asterisk designates a p-value less than 0.05; Three asterisks designate a p-value less than 0.001.